Infections and molecular characterization of anisakid nematodes from two species of marine fish northwest Arabian gulf

The present study provides new insight into valuable information on the diverse structure of anisakid population, discusses limited species richness, and also relationship with other closely diversity-related taxa in NCBI databases Epinephelus diacanthus coioides fish. fishing area consists various locations Arabian Gulf. A total 69 E.coioides E. were examined, (n= 48) infected. Larval stages (n=1,119). Isolated larvae encysted within mesenteries peritoneum viscera fish organs, a prevalence 81.25% infection 59.459 % respectively. Molecular analysis was carried out thirty individuals nematode parasites who have examined morphology showed some appearance differences, by amplifying internal transcribed spacers ITS ITS-1 nuclear rDNA (rDNA) PCR using primer sets NC5/NC2 SS1/NC13R DNA products. Evolutionary analyses conducted MEGA X. based identity percentage GenBank database that they belong to nematodes, particular, nine distinct Hysterothylacium spp. presence same one host may be cause these genetic variations at level, that's what current has recorded. It been found there is an overlap order nitrogen bases between species, this occurs through fertilization process, while rest clean or only few parasites.